IThOS

Primers design

User manual


Genome, select a fasta file :

Strand : Plus Minus

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Advanced Biological Parameters :

General

Primer size min. :
Primer size max. :

Repeat :

GC content min. % :
GC content max. % :

Auto complementarity

Delta G auto max. (cal/mol) :
Delta G3 auto max. (cal/mol) :

Delta G auto size :

Delta G3 auto size :

TM options :

Oligo Tm min. :
Oligo Tm max. :

DNA concentration (nM)
Salt concentration(nM)

Secondary structures (hairpin)

Stem :
Loop :

deltaG length and deltaG stability of 3' and 5' extremity for internal stability :

Extremity 5' size :
Extremity 3' size :

DeltaG5 (cal/mol):

DeltaG3 min. (cal/mol):  
Delta G3 max. (cal/mol):  

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Last modified: Mon March 10 15:29:01 CET 2008