IThOS
Primers design
User manual
Genome, select a fasta file :
Strand :
Plus
Minus
E-mail
Advanced Biological Parameters :
General
Primer size min. :
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
Primer size max. :
20
21
22
23
24
25
26
27
28
29
30
Repeat :
1
2
3
4
5
6
7
8
GC content min. % :
GC content max. % :
Auto complementarity
Delta G auto max. (cal/mol) :
Delta G3 auto max. (cal/mol) :
Delta G auto size :
Delta G3 auto size :
TM options :
Oligo Tm min. :
Oligo Tm max. :
DNA concentration (nM)
Salt concentration(nM)
Secondary structures (hairpin)
Stem :
2
3
4
5
Loop :
3
4
5
6
7
deltaG length and deltaG stability of 3' and 5' extremity for internal stability :
Extremity 5' size :
1
2
3
4
5
6
7
8
Extremity 3' size :
1
2
3
4
5
6
7
8
DeltaG5 (cal/mol):
DeltaG3 min. (cal/mol):
Delta G3 max. (cal/mol):
For any problem or any question don't hesitate to contact the Webmaster :
Webmaster
Last modified: Mon March 10 15:29:01 CET 2008
